Direct 16S rRNA gene sequencing of polymicrobial culture-negative samples with analysis of mixed chromatograms

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Two cases involving polymicrobial culture-negative samples were investigated by 16S rRNA gene sequencing, with analysis of mixed chromatograms. Fusobacterium necrophorum, Prevotella intermedia and Streptococcus constellatus were identified from pleural fluid in a patient with Lemierre's syndrome and Neisseria meningitidis and Escherichia coli were identified from a petechia in a patient with meningococcal disease.
JournalJournal of Medical Microbiology
Issue numberPt 4
Pages (from-to)486-8
Number of pages2
StatePublished - 1 Apr 2010