Circulating microRNA expression profiles associated with systemic lupus erythematosus

Research output: Contribution to journalJournal articleResearchpeer-review

  • Anting Liu Carlsen
  • Aaron J Schetter
  • Christoffer Nielsen
  • Christian Lood
  • Steen Knudsen
  • Anne Voss
  • Curtis C Harris
  • Thomas Hellmark
  • Mårten Segelmark
  • Søren Knak Jacobsen
  • Anders Bengtsson
  • Niels Henrik Helweg Heegaard

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OBJECTIVE: To evaluate the specificity of expression patterns of cell-free, circulating microRNAs in systemic lupus erythematosus (SLE). METHODS: Total RNA was purified from plasma and 45 different specific mature microRNAs were determined using quantitative reverse transcription polymerase chain reaction assays. A total of 409 plasma samples from 364 different SLE patients, healthy controls, and controls with other autoimmune diseases were included. The results of the primary cohort of 62 SLE and 29 healthy controls were validated in two independent cohorts with a total of 69 SLE, 114 healthy controls, 38 vasculitis, 18 rheumatoid arthritis, and 20 immunosuppressed patients. RESULTS: Seven microRNAs were statistically significantly differentially expressed in SLE plasma. Increased expression was found for miR-142-3p and miR-181a while miR-106a, miR-17, miR-20a, miR-92a, and miR-203 were decreased. Also, miR-342-3p, miR-223, and miR-20a were significantly lower in SLE patients with active nephritis. A predictive model for SLE based on 2 or 4 microRNAs differentiated SLE from controls (76% accuracy) when validated independently (p
LanguageEnglish
JournalExcerpta Medica. Section 31: Arthritis and Rheumatism
Volume65
Issue number5
Pages (from-to)1324-1334
ISSN0014-4355
DOIs
Publication statusPublished - 2013