Haptoglobin-related protein is a high-affinity hemoglobin-binding plasma protein

Research output: Contribution to journalJournal articleResearchpeer-review

  • Marianne Jensby Nielsen
  • Steen Vang Petersen
  • Christian Jacobsen
  • Claus Oxvig
  • David Rees
  • Holger Jon Møller
  • Søren Kragh Moestrup

View graph of relations

Haptoglobin-related protein (Hpr) is a primate-specific plasma protein associated with apolipoprotein L-I (apoL-I)-containing high-density lipoprotein (HDL) particles shown to be a part of the innate immune defense. Despite the assumption hitherto that Hpr does not bind to hemoglobin, the present study revealed that recombinant Hpr binds hemoglobin as efficiently as haptoglobin (Hp). However, in contrast to Hp, Hpr did not promote any high-affinity binding to the scavenger receptor CD163. Binding of hemoglobin to circulating native Hpr incorporated into the HDL fraction was indicated by hemoglobin-affinity precipitation of plasma Hpr together with apoL-I. In conclusion, plasma has 2 high-affinity hemoglobin-binding haptoglobins instead of one, but only Hp-hemoglobin complexes are efficiently recognized by CD163. Circulating Hpr-bound hemoglobin should therefore be taken into consideration when measuring "free" plasma hemoglobin. Furthermore, Hpr-bound hemoglobin might contribute to the biologic activity of the circulating apoL-I/Hpr-containing HDL particles.

LanguageEnglish
JournalBlood
Volume108
Issue number8
Pages (from-to)2846-9
Number of pages4
ISSN0006-4971
DOIs
Publication statusPublished - 2006

    Research areas

  • Amino Acid Sequence, Antigens, Neoplasm, Blood Proteins, Chromatography, Affinity, Haptoglobins, Hemoglobins, Humans, In Vitro Techniques, Protein Binding, Recombinant Proteins, Surface Plasmon Resonance